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PGK1 and p27/kip1 as Biomarkers for Intracellular Redox Status
Author(s) -
Kim JongSang,
Quintos Lesley,
Lee In Ae,
Lim Ji Sun,
Seo Ji Yeon,
Ha Young Ran,
Kim Hak Ryul,
Sung Mi Kyung
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.890.21
Subject(s) - delphinidin , hydrogen peroxide , antioxidant , chemistry , downregulation and upregulation , western blot , catalase , biochemistry , intracellular , microbiology and biotechnology , biology , cyanidin , gene
In this study we investigated the effect of delphinidin, a well‐known antioxidant present in pigmented fruits and vegetables, on the expression of not only PGK1 but also p27/kip1, one of the proteins induced in HT29 colon carcinoma cells treated with delphinidin, in whole animal model. ICR mice were injected with varying concentrations of hydrogen peroxide, delphinidin and both. Western blot analysis for the mouse large intestinal tissue showed that PGK1 expression was increased by hydrogen peroxide in a dose dependent manner but was attenuated by combined treatment with hydrogen peroxide and delphinidin. On the other hand, the expression of p27/kip1 was induced by both hydrogen peroxide and delphinidin in mouse organs. Plasma FRAP levels of delphinidin‐treated mice were decreased significantly as compared to those of hydrogen peroxide‐treated control group, maybe due to compensatory downregulation of endogenous antioxidants and/or relevant enzymes. In conclusion, our data suggest that the expression of PGK1 and p27/kip1 are sensitively responding to the presence of either oxidant or antioxidant.