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Organochlorine Compounds are Absorbed via Lymph and Portal Circulation
Author(s) -
Jandacek Ronald J.,
Tso Patrick
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.889.15
Subject(s) - lymph , chemistry , lymphatic system , enterohepatic circulation , hexachlorobenzene , absorption (acoustics) , portal vein , chylomicron , medicine , physiology , metabolism , pathology , biochemistry , organic chemistry , physics , acoustics , pollutant , lipoprotein , very low density lipoprotein , cholesterol
The metabolism and toxicity of organochlorine compounds (OCs) have been studied extensively. Their persistence in the biosphere and their threat to health continue to be the focus of policy and scientific investigation. In spite of efforts to limit their use, OCs continue to enter into human tissues from the diet. Little is known about the absorption of these materials from the intestine. The route of absorption can determine whether or not peripheral tissues are exposed to parent compound or to metabolites from the liver. Lipophilic compounds are principally incorporated into chylomicrons and transported in lymph, and hydrophilic compounds are principally absorbed by the portal vein. We have developed an animal model which allows the simultaneous determination of the absorption of OCs by each route. The portal vein of a rat is catheterized to allow both normal flow and periodic sampling of dietary substances. A second catheter in the mesenteric lymph duct to divert intestinal lymph, allows measurement of dietary compounds in chylomicrons. We used this model to study hexachlorobenzene and DDT. The data are consistent with absorption of DDT and hexachlorobenzene by both routes. Both compounds appear in the lymph at a slower rate than triolein. There is significant absorption of both compounds by the portal vein, as shown by analysis of portal plasma and tissue analyses of the animals. Funded by ES014464 ‐A1(NIH/ESO)