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Profiling of gene expression and proteomics in skin tissues of weight controlled mice via exercise and/or calorie restriction
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.886.8
Subject(s) - calorie restriction , gene expression , proteomics , gene , fold change , biology , body weight , gene expression profiling , endocrinology , medicine , calorie , bioinformatics , genetics
Experimental evidence has demonstrated that weight control via dietary caloric restriction and/or exercise may prevent cancer. However, the underlying mechanisms are not clear. In this study, we randomly assigned SENCAR mice into 4 groups for 10 wks: ad libitum‐fed sedentary control, ad libitum‐fed exercise (AE), exercise but pair‐fed at the amount of control (PE), and 20% of dietary calorie restriction (DCR). Two hrs after topical TPA treatment, the skin tissues were analyzed by Affymetrix for gene expression and DIGE for proteomics, respectively. The body weights were significantly reduced in both DCR and PE but not AE mice when compared with the control. Among 39,000 transcripts, 1781, 1058, and 706 genes were found significantly changed by DCR, PE, and AE, respectively. PE and DCR showed similar impact on signaling pathways‐related gene expression as analyzed by GenMAPP. Braf in MAPK pathway and Akt1 in insulin signaling pathway appeared to be the key targets in response to weight change. Of the total 120 proteins identified, about 27 proteins were significantly changed by DCR. The correlation between gene and protein expression and their interaction to TPA‐induced cancer risk are further examined through Cytoscape and GenMAPP. Overview of all the gene and protein alterations may provide intrinsic mechanisms of cancer prevention by weight control (supported by NIH R01CA106397).