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Adequate dietary calcium restores vertebral trabecular bone microarchitecture and strength and improves femur calcium concentration following calcium depletion in young female rats
Author(s) -
Hunt Curtiss D,
Stoecker Barbara J
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.883.1
Subject(s) - calcium , femur , weaning , medicine , endocrinology , chemistry , diaphysis , low calcium , osteoporosis , zoology , biology , surgery
To determine whether dietary calcium deficiency during adolescence permanently reduces lifetime potential to attain peak bone mass and strength, female Sprague‐Dawley rats were separated into groups (n=10) and fed an AIN‐93G‐based diet containing 20% (1000 mg Ca/kg) of the calcium requirement from weaning until 105 d of age (DEF); switched near sexual maturity (63 d of age) to 100% of the requirement (5000 mg Ca/kg) (RP); or pairfed (5000 mg Ca/kg) throughout to group RP (PF). At 105 d of age, Ca repletion restored L4 trabecular bone volume fraction (RP: 27.0 ± 1.1%; PF: 28.1 ± 1.2%; p = 0.46) and strength (estimated by force to compression with finite element analysis) (RP: 11.51 ± 0.87 N; PF: 11.9 ± 1.42 N; p =0.83). In femurs, Ca repletion increased (DEF: 176 ± 1; RP: 214 ± 3; p < 0.0001) but did not completely restore (RP: 214 ± 3; PF: 243 ± 8 mg Ca/g; p < 0.0001) femur calcium concentration. However, cortical thickness (measured at midshaft of femur diaphysis) was completely restored by Ca repletion (RP: 0.6; PF: 0.6 mm; p = NS). Findings from this animal model suggest that the deleterious effects of calcium deficiency during adolescence can be reversed with proper dietary Ca intakes.