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In vivo and in vitro regulation of adipogenesis by caffeine in yerba mate tea in comparison to other caffeine sources
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.881.7
Subject(s) - caffeine , in vivo , adipogenesis , in vitro , chemistry , endocrinology , medicine , adipocyte , body weight , biology , adipose tissue , biochemistry , microbiology and biotechnology
Caffeine and yerba mate tea (MT) have been associated with body weight regulation. The objective of this study was to examine the effectiveness of caffeine from yerba mate tea (MTc) on adipogenesis in comparison to caffeine from coffee (Cc) and synthetic caffeine (C), in vitro and in vivo . Swiss 3T3‐L1, preadipocytes and adipocytes, were used to determine proliferation through cell viability and Oil Red O assays. MT, MTc, and Cc caused a dose‐dependent inhibition on preadipocyte proliferation (IC 50 = 28 mM eq. to chlorogenic acid, IC 50 = 48 mM, IC 50 = 115 mM, respectively). MTc and Cc showed an inhibitory mechanism on preadipocyte proliferation that occurred via preadipocyte factor‐1 (Pref‐1) while adiponectin levels on mature adipocytes were increased by two‐fold from the control. A significant decrease in number and size of mature adipocytes was observed in MTc and Cc treated cells showing an inhibitory mechanism on maturation of adipocytes from the control. Male Sprague‐Dawley rats fed a high fat diet that contained MT, MTc, Cc and C at 0.1% showed that only MTc, Cc and C produced the lowest body fat accumulation (9.6 ± 0.9%, 10.1 ± 1.5%, 9.3 ± 0.8%, respectively) versus the control (12.3 ± 1.0%), p < 0.05. Body weight was only affected by the presence of MTc (145.2 ± 5.8 g) and C (136.1 ± 16.4 g) versus the control (162.0 ± 13.7 g), p < 0.05. In vitro and in vivo results suggest that MTc and Cc are potential regulators in adipogenesis.