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Activation maps in the nucleus of the solitary tract (NTS) in response to internal stimuli
Author(s) -
Schwarz Jessica,
Darcel Nicolas,
Rampin Olivier,
Andrey Philippe,
Burguet Jasmine,
Fromentin Gilles,
Tomé Daniel,
Maurin Yves
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.878.5
Subject(s) - solitary tract , brainstem , solitary nucleus , nucleus , sensory system , anatomy , stimulus (psychology) , biology , coronal plane , neuroscience , psychology , psychotherapist
During digestion, integration of gut information by the NTS is involved in the induction of satiation. Little is known about the encoding of peripheral information by the NTS. Similarly to other sensory encoding processes, internal sensing may rely on stimulus specific activation patterns. The aim of this study was to search for such patterns elicited in the NTS by intragastric loads of macronutrients. Male C57BL/6J mice were gavaged with isocaloric loads of either trioleate or saccharose or peptone. Animals were perfused and their brains collected and postfixed. Serial coronal brainstem sections (20 μm thick) covering the entire caudo‐rostral extent of the NTS were collected. Immunodetection of Fos protein and luxol fast blue / neutral red staining were performed on odd‐ and even‐numbered sections, respectively. Digitized brainstem images (20x) were acquired. Using Free‐D, a 3D‐reconstruction software, the contours of the brainstem and of the NTS were delineated and the Fos‐positive neurons pointed. After registration, the piling‐up of the segmentations yielded three‐dimensional models of the NTS including the activated neurons. The activation maps are compared.