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The effect of substrate supply in modulating plasma Glutathione (GSH) levels in HIV+ treated patients
Author(s) -
Burini Roberto Carlos,
Pereira Paulo Câmara Marques,
BorgesSantos Maria Dorotéia,
Yu YongMing
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.869.18
Subject(s) - glutathione , uric acid , medicine , chemistry , endocrinology , homocysteine , albumin , cysteine , vitamin c , biochemistry , enzyme
HIV+ patients usually present low levels of GSH and its precursors. PURPOSE: To investigate the responses of GSH and Sulfur amino acid (SAA)pathway to dietary supplements of N‐acetyl‐cysteine (NAC) and Gln. METHODS:12 HIV+ (25–36 yrs) patients (G2) under HAART and 20 (23–28 yrs) healthy controls (G1) were assessed at baseline for anthropometry and plasma biochemistry, and randomly assigned to two diets containing NAC (1g/d) or Gln (20g/d) supplementation. Blood samples were drawn before and by the end of the 7‐day diets to assay the plasma levels of SAA, GSH/GSSG, Ser, Gly, Glu and Gln. The effects of diets and HIV+ were analysed statistically (p=0.05). RESULTS: The measured BMI, albumin, vitamin B12, HDL‐cholesterol and Hcy were similar between G1 and G2. G2 showed higher values of glucose, triglycerides, and GSSG, but lower concentrations of uric acid, folic acid, and GSH. NAC diet significantly increased SAA and GSH in G2 but only Tau and GSH were comparable to G1. Gln supplementation also increased GSH comparable to G1, so did Gly. CONCLUSIONS: GSH deficiency in HIV+ patients can be replenished by dietary supplement of either NAC or Gln. Supported by FAPESP, CNPq.