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Impairment of skin wound healing in mice fed with glutamate free diets: evidences for nutritional significance of dietary glutamate
Author(s) -
Yoneda Junya,
Kawamata Yasuko,
Chin Keigi,
Torii Kunio,
Sakai Ryosei
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.869.17
Subject(s) - glutamate receptor , monosodium glutamate , amino acid , wound healing , glutamic acid , ingestion , sodium glutamate , medicine , chemistry , biology , endocrinology , biochemistry , immunology , receptor , raw material , organic chemistry
Background: Glutamate is one of the most abundant amino acids in food proteins. It has been shown that dietary glutamate plays crucial role as source of intestinal energy and so on, however, nutritional significance of dietary glutamate for whole body is not recognized well, because dietary glutamate is exclusively metabolized in the gut. Objective: The aim of this study is to investigate the nutritional significance of dietary glutamate in the whole body by using wound model mice Method: Male Balb/c mice (6–0 weeks) were fed either control or glutamate‐free diets for 16 days. Seven days after the start of feeding, the mice were inserted 2 sponges into individual subcutaneous pockets and sacrificed 9 days after the operation. Results: Mice fed glutamate‐free diets displayed delayed cutaneous wound healing compared with mice fed control diet, which was recovered by ingestion of 2% monosodium glutamate (MSG) in tapping water. The delay was characterized by decrease in cytokine levels, lymphocyte recruitment to the wound site, and granulation. The delay of healing was associated with decreased local and systemic concentration of essential amino acids, such as BCAA and Phenylalanine. Conclusion: These results suggested that dietary glutamate substantially contributes to the promotion of normal wound healing presumably by maintaining certain amino acid levels in the body.