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Control materials for validating measurement of vitamin D in key foods for the USDA National Food and Nutrient Analysis Program (NFNAP)
Author(s) -
Holden Joanne M,
Byrdwell William Craig,
Exler Jacob,
Harnly James,
Hollick Michael,
Hollis Bruce,
Horst Ronald L,
Patterson Kristine,
Phillips Katherine,
Wolf Wayne
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.868.10
Subject(s) - food science , food standards , orange juice , nutrient , vitamin , certified reference materials , environmental science , microbiology and biotechnology , mathematics , chemistry , food safety , biology , statistics , biochemistry , organic chemistry , detection limit
Data for vitamin D in the National Nutrient Database for Standard Reference are being updated. High priority foods including fish and vitamin D fortified orange juice, breakfast cereals, milk, American cheese, and yogurt were sampled (n=446) according to a nationwide plan, to be analyzed by qualified laboratories. A lack of certified reference materials required development of control materials to ensure accurate measurements. Matrix‐specific food homogenates (e.g. salmon, orange juice) were prepared and analyzed by six laboratories experienced in determining Vitamin D. Initial results showed wide disparity among the laboratories (CV = 26–46%). Subsequent recovery studies, mass spectrometric analysis, verification of standards, optimization of extraction solvents resulted in reliable values. The three labs chosen for future analyses obtained statistically similar results. These data were used to assign mean values and tolerance limits for four of the control materials. Due to inconsistent data for salmon, further work is required to validate those results. This research was supported by USDA, NIH, and the Beverage Institute for Health & Wellness (Coca‐Cola).