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Gene expression of mesenchymal stem cell (MSC) involved in the regeneration of injured liver tissue
Author(s) -
Cho KyungAh,
Ju SunYoung,
Ryu KyungHa,
Woo SoYoun
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.862.25
Subject(s) - mesenchymal stem cell , biology , microbiology and biotechnology , liver regeneration , regeneration (biology) , stem cell , cell type , cell , cancer research , genetics
Mesenchymal stem cells (MSCs) are potential cell source for the development of therapeutic product. Recent studies reported that MSCs transplantation to the damaged organ including heart, liver and kidney result in restore of damaged tissue. Because it is not clearly revealed the mechanism involved in the regeneration process, we focused the therapeutic potential of MSCs in injured liver, and investigated the gene expression profiles of MSCs in presence of injured liver cell and normal liver cell using a microarray containing 40,000 genes. For inducing liver cell regeneration, we co‐cultured MSCs with CCl 4 induced injured liver cell and normal liver cell, respectively. The cells were obtained from C57BL6 female mice. After 48 hrs of coculturing, MSCs were collected and RNA was extracted for microarray analysis. The data were normalized with 8 categories such as signal transduction, cell cycle, response to stress and immune response. Expressed genes were various under each conditions. For example, in presence of injured liver cells, MSCs highly expressed several genes involved in angiogenesis including fibroblast growth factor 1, prostaglandin‐endoperoxide synthase 2 and Tenascin C. Genes involved in cellular physiolosical process such as TYSP‐like 3, sentrin specific peptidase 6, activin A receptor type IC, intestinal cell kinase and pantothenate kinase also highly expressed. Our research may provide information on the differential molecular mechanisms regulating the MSC properties in regeneration of injured liver tissue.