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Yersinia pestis elicits growth‐condition dependent patterns of dendritic cell IL12p40 production and CCL19‐dependent chemotaxis
Author(s) -
Robinson Richard T.,
Khader Shabaana A.,
Smiley Stephen T.,
Cooper Andrea M.
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.860.13
Subject(s) - yersinia pestis , chemotaxis , biology , microbiology and biotechnology , ccl19 , immune system , chemokine , immunology , gene , genetics , virulence , receptor , chemokine receptor
Yersinia pestis is the causative agent of bubonic plague. At the temperature of Y. pestis natural flea vector (26°C), Y. pestis expresses a profile of genes and lipopolysaccharide variants that are distinct from those expressed in a mammalian host (37°C). As dendritic cells (DCs) are targeted by Y. pestis, we examined whether DC production of IL‐12p40 ‐ a critical component of both cellular and humoral immunity ‐ was differentially stimulated by Y. pestis grown at 26°C versus Y. pestis grown at 37°C (hereafter referred to as Y.pestis‐26° and Y. pestis‐37°, respectively). Using IL12p40‐reporter bone‐marrow derived DCs (BMDCs) we demonstrate that Y.pestis‐26° is capable of eliciting a >25‐fold increase in IL12p40 production above unstimulated controls in a dose‐dependent manner. This IL12p40 response, however, was much attenuated among BMDCs stimulated with Y. pestis‐37°. As IL12p40‐homodimer contributes to DC chemotaxis, we also examined the chemotactic ability of Y.pestis‐26° versus Y. pestis‐37° stimulated BMDCs. Stimulation of BMDCs with Y.pestis‐26° significantly increased their chemotaxis towards CCL19 in a dose‐dependent manner, whereas the CCL19‐dependent chemotaxis of Y. pestis‐37° stimulated BMDCs was only minimally increased. Thus, Y.pestis‐26° and Y. pestis‐37° differentially stimulate BMDCs, with the Y. pestis‐37° being associated with diminished IL12p40‐production and chemotaxis.