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T‐cell responses to influenza vaccination among patients with heart failure
Author(s) -
Moran John Joseph McDonald,
Hayney Mary S.,
Sweitzer Nancy K.,
Vardeny Orly
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.857.16
Subject(s) - medicine , peripheral blood mononuclear cell , influenza vaccine , antigen , vaccination , immunology , immunization , t cell , sensitization , immune system , in vitro , biology , biochemistry
Purpose: The objective of this study was to measure T cell response in patients with heart failure (HF) compared to healthy controls (HC) following influenza vaccination using the trans vivo delayed‐type hypersensitivity (DTH) assay, which mimics physiologic conditions. Methods: Eighteen HF subjects and 16 HC were immunized with influenza vaccine. Peripheral blood mononuclear cells (PBMC) were isolated from blood drawn 4–6 weeks post immunization. PBMC were mixed with influenza vaccine antigens (H1N1, H3N2, and B), and injected into the footpads of SCID mice. The resulting swelling is an index of human T cell sensitization. To detect a difference of 50 μm (α=0.05 and power = 95%) 15 per group were needed. Results: All subjects mounted an antibody response to the influenza vaccine. T cell responses to the B antigen were similar between the HF and HC groups (75±8 vs. 66±9 μm; p=0.49). Responses to the newest vaccine antigen, H3N2, were less vigorous in the HF participants compared with the HC response (mean 65±8 vs. 89±6 μm; p=0.031; t test), and there was a trend toward a reduced response to the H1N1 antigen by the HF group (mean 58±7 vs. 81±10 μm; p=0.06). Conclusions: HF pts demonstrated reduced T cell responses to influenza vaccine, due to lower response to the newest vaccine antigen compared with HC. Lower T cell responses may be a reason that HF patients are at increased risk for influenza and its complications. Funded by the NIH.