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Changes in Lung Antigen Presenting Cell Populations in a Mouse Model of Influenza Infection
Author(s) -
Ho Adrian WS,
Kemeny David Michael
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.857.12
Subject(s) - cd11c , immunology , integrin alpha m , lung , antigen presenting cell , dendritic cell , antigen presentation , immune system , t cell , biology , macrophage , antigen , acquired immune system , medicine , in vitro , biochemistry , gene , phenotype
Antigen preseting cells (APCs) in the lung serve as sentinels against airborne pathogens. Infection of the respiratory tract by Influenza A virus induces a recruitment of APCs to the lung. Of the various populations of APCs, lung dendritic cells play an essential role in the induction of adaptive immune responses to pulmonary infection. Using a mice model of influenza, we report the changes in the lung APC populations that occur over time. We focused our analysis on three groups of APCs, the CD11c+CD11b + MHCII + lung dendritic cell, the CD11c + CD11b − MHCII low/int alveolar macrophage and the CD11c + CD11b − MHCII high cell, which remains relatively uncharacterised in literature. We note a significant increase in the number of CD11c + CD11b + MHCII + cells over time, but not in the other two populations. This is despite the detection of high CCL2 levels in brochoalveolar lavage. Finally, we show that lung dendritic cells upregulate expression of co‐stimulatory molecules even before migration to lymph nodes draining the lung, where co‐stimulatory molecule expression is further enhanced.