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mPDCA‐1 Ag reveals a novel antigen presenting cell modulating Th2 type immune responses against influenza virus infection
Author(s) -
Yoo JaeKwang,
Fish Eleanor
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.857.10
Subject(s) - cd11c , biology , t cell , influenza a virus , immune system , population , virus , antigen , antigen presenting cell , t cell receptor , immunology , virology , phenotype , medicine , biochemistry , environmental health , gene
Objectives: Here, we have identified a novel immune cell population expressing mPDCA‐1 Ag in lymphoid tissues, which are mPDCA1 + CD11c − TcRß − B220 − . Studies were conducted to investigate the in vivo function of this cell population in the context of influenza virus infection. Methods: B6 mice were infected with A/WSN/33 influenza virus by intranasal inoculation. The subsequent cellular responses of the mPDCA1 + CD11c − TcRß − B220 − cells were monitored by FACS‐analysis. Results: Study revealed that these cells are APCs responding to viral infection. Pulmonary infection with A/WSN/33 virus stimulated these cells to up‐regulate the surface expression of MHC‐II and co‐stimulatory molecules. Influenza virus infection induced the migration of this cell into infected lungs, spleen and DLN. In the DLN, mPDCA1 + CD11c − TcRß − B220 − cells presented viral antigens to CD4 + T cells. Influenza virus activated mPDCA1 + CD11c − TcRß − B220 − cells also produced cytokines including IL‐25, IL‐2 and IL‐4 in lungs and DLN. Notably, ex vivo studies revealed that influenza virus activated mPDCA1 + CD11c − TcRß − B220 − cells induced the generation of Th2 effector T cells from antigen primed CD4 + T cells in the DLN. Conclusion: These results suggest that a newly described mPDCA1 + CD11c − TcRß − B220 − cell population may regulate a Th2 type immune response against pulmonary influenza virus infection.