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HIV‐1‐Infected Peripheral Blood Mononuclear Cells (PBMC) Promote Neutrophil Survival and Induce GM‐CSF‐Dependent Expression of HLA‐DR and IFN‐γ‐Dependent Expression of CD64
Author(s) -
Fu Jun,
Riggs Emily,
Thomas Larry
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.856.25
Subject(s) - peripheral blood mononuclear cell , cd64 , cd86 , antibody , immunology , cd80 , immune system , biology , microbiology and biotechnology , virology , cd40 , t cell , in vitro , cytotoxic t cell , biochemistry
We examined the ability of HIV‐1 infected PBMC to influence neutrophil function. Incubating neutrophils with supernatant of PHA‐activated PBMC that had been cultured with HIV‐1 for six days (HIV‐1 + culture supernatant) significantly increased neutrophil survival at 48 hr. Depletion of the HIV‐1 virions by centrifugation did not alter the pro‐survival activity of the HIV‐1 + culture supernatant. The HIV‐1 + culture supernatant also induced expression of HLA‐DR and CD64 by neutrophils. The induction of HLA‐DR paralleled induction of HLA‐DR expression by GM‐CSF. The HIV‐1 + culture supernatant, however, did not induce neutrophil expression of CD80, CD86, or CD40. The HIV‐1 + culture supernatant stimulated concentration‐dependent increases in neutrophil HLA‐DR and CD64 at supernatant concentrations containing 5 to 50 pg GM‐CSF/ml and 3 to 30 pg IFN‐γ/ml, respectively. Neutralizing anti‐GM‐CSF antibody inhibited HLA‐DR expression and neutralizing anti‐IFN‐γ antibody inhibited CD64 expression induced by the HIV‐1 + culture supernatant. These results indicate that HIV‐1‐infected PBMC can prolong neutrophil survival and potentially influence neutrophil immune activity through the production of GM‐CSF and IFN‐γ. Supported by NIH Grant R21 DE070170