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BG1, a candidate gene affecting the occurrence of herpesvirus–associated avian lymphomas
Author(s) -
Wang Yujun,
Goto Ronald M.,
Miller Marcia M.
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.856.10
Subject(s) - biology , genetics , gene , allele , locus (genetics) , haplotype , untranslated region , population , congenic , three prime untranslated region , microbiology and biotechnology , messenger rna , demography , sociology
Oncogenic herpesvirus infections contribute to the occurrence of lymphomas in the human population. Genetic makeup may have a role in susceptibility. Through genetic mapping with recombinant haplotypes we have identified BG1 , a member of the extended butyrophilin gene family, as a gene having a significant role in MHC‐associated resistance to the occurrence of T‐cell lymphomas that often follow infection with gallid herpesvirus 2 in the chicken animal model. Significant differences in tumor incidence occur among congenic birds differing only at the BG1 locus. Two alleles, BG1*R2 and BG1*R4 , are respectively associated with tumor resistance and susceptibility. The alleles differ only in the 3′‐untranslated region (3′‐UTR) with the longer 3′‐UTR of the BG1*R4 allele containing unique sequence. In dual Firefly/Renilla luciferase reporter assays we found that the 3′‐UTR of the BG1*R4 suppresses PMA‐stimulated translation of the firefly reporter gene, while the 3′‐UTR of BG1*R2 has a far less inhibitory influence. With further constructs we have narrowed the effective region of the BG1*R4 3′‐UTR to a region relatively rich in CG dinucleotides. Supported in part by NCI R21 CA105426.