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Retinal laser burn alters immune regulation of the eye
Author(s) -
Lucas Kenyatta G.,
Qiao Hong,
SteinStreilein Joan
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.854.9
Subject(s) - immune privilege , immune system , medicine , immunology , immune tolerance , uveitis , inflammation , antigen , ophthalmology
It is known that immune privilege allows for immune protection in the eye in the absence of inflammation. Only a few events have been reported to overcome the immune privileged mechanisms in the eye. Here we report the effect of retinal laser burn (RLB) on immune privilege in the anterior chamber. As early as 6hr post RLB and as late as 21D, antigen inoculation into the anterior chamber failed to induce ACAID induction in both the eye that received the RLB and the contralateral eye. Aqueous humor samples harvested from naïve eyes but not from either the burned and the non‐burned eye (24 hrs post RLB) were able to down regulate the expression of CD40 or up regulate IL‐10 mRNA in peritoneal exudates cells (PEC) and convert them to tolerogenic antigen presenting cell (APC). RTPCR analyses of mRNA extracted from experimental and control eyes, showed little or no change in TGFβ. Increasing use of lasers in both industrial and medical fields raises the risk of losing immune regulation in the eye and adds the subsequent risk of being a candidate for autoimmune uveitis. This work was supported in part by grants to JSS: DOD W81XWH and NIH EY11983; KL: NIH T32 EY07145.