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Dietary fat modulates intestinal absorption of luminal gut antigens
Author(s) -
Eckhardt Erik,
Ghoshal Sarbani,
Ward Martin,
Woodward Jerold,
Villiers Willem
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.854.8
Subject(s) - ovalbumin , chylomicron , mesenteric lymph nodes , chemistry , immune system , antigen , triolein , lymph , spleen , tributyrin , endocrinology , microbiology and biotechnology , biology , biochemistry , immunology , medicine , pathology , lipoprotein , very low density lipoprotein , cholesterol , lipase , enzyme
BACKGROUND: Mesenteric lymph nodes (MLN) are important for proper immune responses to gut antigens. Dietary long‐chain fat stimulates mesenteric lymph flow and lymphatic secretion of chylomicrons (CM) which have been shown to bind lipopolysaccharides (LPS) and amphiphilic peptides. HYPOTHESIS: CM formation promotes intestinal absorption of LPS and peptidic antigens. METHODS: Antigen absorption was measured in mice and CaCo‐2 cells, absorbed ovalbumin (OVA) was detected by adoptive T‐cell (DO11.10) transfer. RESULTS: Fasted mice gavaged with LPS absorbed 3‐times more LPS into blood and MLN when subsequently gavaged with triolein than with tributyrin. This was abolished by an inhibitor of CM formation, Pluronic L‐81. Absorbed LPS was associated with plasma CM remnants. CaCo‐2 cells pretreated with LPS secreted more LPS from cell‐associated pools into basolateral medium when stimulated with oleic acid than with butyric acid; this was also Pl‐81 sensitive. MLN P38 MAPK phosphorylation was higher after gavage with triolein than with tributyrin. Balb/C mice adoptively transfered with CFSE‐labeled DO11.10 CD4 T‐cells and gavaged with OVA and triolein had markedly more CFSE‐labeled cells in MLN and spleen than mice gavaged with OVA and tributyrin; T‐cell proliferation was also elevated. CONCLUSION: Dietary fat may affect the intestinal immune system by modulating absorption and MLN delivery of gut antigens.