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T helper dependent CTL migration into the vaginal mucosa
Author(s) -
Nakanishi Yusuke,
Lu Bao,
Gerard Craig,
Iwasaki Akiko
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.852.5
Subject(s) - ctl* , immunology , biology , chemokine , virus , cytotoxic t cell , secretion , lymph node , virology , immune system , cd8 , in vitro , biochemistry
Cytotoxixc T lymphocyte (CTLs) play a critical role in controlling virus infection. However, little is known regarding how CTLs migrate into the mucosal tissues and mediate antiviral host defense. Here, we addressed the mechanism of CTL migration into the mucosal site. By using mouse model of vaginal herpes simple virus (HSV) 2, we demonstrated that HSV‐specific CTLs were localized in the vaginal epithelia and migration of HSV‐specific CTLs depends on the presence of CD4 help. In addition, although division and IFN‐γ production by HSV‐specifc CTLs in the draining lymph node normally occurred in the absent of CD4 help, there was a decrease in the accumulation of these cells in the helpless mice. In the absence of CD4 T help, secretion of IFN‐γ and IFN‐inducible chemokines were diminished at the infection site. Importantly, HSV‐specific CTLs deficient in the chemokine receptor CXCR3 failed to migration into the vaginal mucosa after infection. These observations indicated that CD4 help is required to support CTL migration to the site of infection by inducing appropriate chemokines secretion in situ .