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Requirements for cell lineage‐restricted expression of MHC class II in the development of CD4 T cell‐dependent immunity in the intestine
Author(s) -
Perrigoue Jacqueline Gage,
Allenspach Eric,
Laufer Terri,
Artis David
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.851.2
Subject(s) - mhc class ii , biology , immunology , cd11c , immune system , t cell , lamina propria , dendritic cell , population , mhc class i , immunity , intestinal mucosa , major histocompatibility complex , medicine , epithelium , biochemistry , genetics , environmental health , gene , phenotype
Many models have been proposed for how luminal antigens are sampled by immune cells within the intestinal mucosa ranging from direct sampling via specialized dendritic cell (DC) populations to intestinal epithelial cell (IEC)‐dependent transfer of antigen to the lamina propria. To test whether DC‐restricted expression of MHC class II is sufficient to promote protective CD4 T cell responses in the intestine, mice in which I‐Aβb expression is restricted to CD11c+ DC (CD11c/Aβb) were infected with the intestinal parasite Trichuris, immunity to which is dependent on Th2 cells. Following infection, littermate control mice exhibited Th2 cytokine‐dependent goblet cell hyperplasia and eradication of Trichuris. In contrast, CD11c/Aβb mice developed persistent infection associated with reduced Th2 cytokine production and increased IFN‐γ. These data suggest that in addition to DC, another population of MHC class II expressing cells is required for Th2 cytokine‐mediated immunity in the intestine. Previous studies have implicated IEC‐intrinsic MHC class II expression in intestinal immune regulation and following exposure to Trichuris, EC upregulated surface MHC class II. Identifying the functional significance of IEC‐intrinsic MHC class II expression in regulating CD4 T cell responses may have implications for the design and delivery of mucosal vaccines and treatment of T cell‐mediated inflammatory diseases.