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HOZOT, a novel type of human regulatory T cell lines, showed opposing activities, helper and suppressor, depending on the stimulations of dendritic cells or anti‐CD3 antibody
Author(s) -
Sugimoto Akira,
Suzuki Motoyuki,
TsujiTakayama Kazue,
Yamamoto Mayuko,
Harashima Akira,
Inoue Toshiya,
Otani Takeshi,
Takeuchi Makoto,
Nakamura Shuji,
Kibata Masayoshi
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.848.2
Subject(s) - cd28 , cd3 , cytotoxic t cell , t cell , immune system , microbiology and biotechnology , chemistry , dendritic cell , antibody , biology , immunology , cd8 , in vitro , biochemistry
HOZOT cell lines are one type of the regulatory T cells (Treg) established from human umbilical cord blood (UCB) without cytokines. These HOZOT cells have multi‐functional activities, not only suppressor but also cytotoxic activity. In this study, we have examined another novel function of HOZOT in immune system. Cord blood naive CD4 + T cells as responder cells were stimulated with dendritic cells (DC) or plate coated anti‐CD3 antibody (anti‐CD3 Ab) in the presence of HOZOT‐4. The DC‐induced proliferation of naive CD4 + T cells derived from UCB was inhibited by the addition of HOZOT‐4. Whereas, anti‐CD3 Ab‐induced proliferation was promoted by the same treatment. Anti‐CD3 Ab ‐cultures have produced IFN‐γ, not IL‐4. Opposing effects of HOZOT‐4 were unique because naturally occurring Treg inhibited both DC‐ and anti‐CD3 Ab‐induced stimulations. Kinetics study showed that cell division was first detected at day 3 and increased later on. IL‐2 was not involved in the mechanism of helper activity of HOZOT‐4 because no IL‐2 was detected in the anti‐CD3 Ab‐induced coculture system and blocking antibodies for IL‐2 and IL‐2R did not affect helper activity. Moreover, this helper activity could not be inhibited with blocking reagents for costimulatory molecule of CD28/B7. This helper activity exerted by HOZOT may be mediated through a novel costimulatory pathway.