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Increase in CD4+ / CD25+ / Fox P3+ Regulatory T Cells following chemical sympathectomy with 6‐Hydroxydopamine
Author(s) -
Bhowmick Sourojit,
Singh Anurag,
Thrall Roger S,
O’Rourke james,
Cone Robert E
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.848.10
Subject(s) - hydroxydopamine , il 2 receptor , sympathectomy , spleen , in vitro , chemistry , cytotoxic t cell , endocrinology , immunology , medicine , dopaminergic , dopamine , biochemistry
Ablation of the Sympathetic Nervous System (SNS) by systemic 6‐Hydroxydopamine (6‐OHDA) enhances the production of IgM antibodies, depresses hypersensitivity and the production of CD 8+ Regulatory T Cells. Here we investigated the effect of chemical sympathectomy induced by 6‐OHDA on CD4+ / CD25+ / Fox P3+ Regulatory T Cells (T Regs) in C57BL/6 (B6) mice. Two days after i.p injection of 6‐OHDA there was a significant increase (40–60%) in the number of T‐Regs in the spleen and inguinal lymph nodes as seen by flowcytometry. This increase in T‐Reg number in 6‐OHDA‐ treated mice did not occur in mice that received desipramine, which prevents the uptake of 6‐OHDA by the nerve terminals. However contact sensitivity to trinitrophenol (TNP) could not be induced in 6‐OHDA treated mice. The suppression of anti CD3‐induced in vitro proliferation of T Effector Cells by T Regs from 6‐OHDA‐treated donors was quantitatively equivalent to that from donors receiving PBS. These results suggest that ablation of the SNS can induce or expand systemic T Regs.