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Identification of two early differentiated populations within CD4+ and CD8+ TEMRA T‐cells
Author(s) -
Derhovanessian Evelyna,
Koch Sven,
Haehnel Karin,
Pawelec Graham
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.846.10
Subject(s) - cd28 , biology , cd8 , population , t cell , cytotoxic t cell , microbiology and biotechnology , immunology , antigen , immune system , medicine , in vitro , genetics , environmental health
Antigen‐experienced T‐cells can be divided into functionally distinct populations, according to the expression of different cell surface markers, such as CD45RA, CCR7, CD27 and CD28. Effector T‐cells re‐expressing CD45RA and lacking the chemokine receptor CCR7 (TEMRA cells), are considered to be late differentiated cells, with limited proliferative but high functional capacity. Using 10‐color flow cytometry, we subdivided circulating CD4+ and CD8+ effector memory (EM) (CCR7−, CD45RA−) and TEMRA (CCR7−, CD45RA+) cells of healthy individuals and cancer patients according to their surface expression of CD27 and CD28, as well as CD57, a marker up‐regulated on very late‐differentiated T‐cells. In both cohorts, we identified two subsets of early differentiated CD27+CD28+ and CD27+CD28‐lymphocytes, with very low CD57 expression in both the CD4+ and CD8+ TEMRA population, whereas most of the CD28−CD27− TEMRA cells were strongly positive for this putative marker of T cell senescence. Further functional analysis, combined with cell surface phenotyping showed that these two CD27+ TEMRA populations represent early‐differentiated T‐cells with higher proliferative capacity and lower IFN‐γ secretion compared to the late differentiated CD28−CD27− TEMRA cells. This work was supported by EC‐LSHG‐CT‐2007–036894 “LifeSpan” and DFG‐SFB685‐B4.