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Human red blood cells (RBCs) labeled at discrete 5 densities can be used to independently determine blood volume in vitro
Author(s) -
MatthewsMock Nell I.,
Widness John A.,
Schmidt Robert L.,
Mock Donald M.
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.844.8
Subject(s) - biotinylation , in vivo , flow cytometry , population , in vitro , blood volume , chemistry , volume (thermodynamics) , biotin , cytometry , avidin , human blood , microbiology and biotechnology , chromatography , biology , biochemistry , medicine , genetics , physiology , physics , environmental health , quantum mechanics
We have previously shown that blood volume can be independently and simultaneously measured in vivo using 2 discrete densities of biotinylated RBCs (2004, Mock, et al. Transfusion 44: 431). We further have shown that we can label RBCs at up to 5 discrete densities (2005, Mock and Mock, FASEB J 19:A260). Here we sought to determine whether human RBCs biotinylated at 5 densities can be used to independently determine blood volume in vitro. We used the biotinylation reagent s‐NHS‐biotin in increasing amounts from 5.8 to 634 μg/mL of RBC to biotinylate RBC surface proteins; each population of RBCs was enumerated by flow cytometry after complexing with fluorescein‐labeled avidin. Mean error in blood volume was 4%, SD = ±7%. These data provide evidence that blood volume can be accurately measured simultaneously and independently using up to 5 biotin densities. We speculate that different densities of biotinylated RBCs can be used to determine human blood volume in vivo at intervals over time. Further, we speculate that discrete populations of labeled RBCs can be used to determine survival of multiple populations of transfused RBCs. This work was supported by NIH Program Project Grant HD 046925.