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Transcriptomics and proteomics of drought tolerance in peanuts
Author(s) -
Kottapalli Kameswara Rao,
Burrow Mark D,
Puppala Naveen,
Payton Paxton
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.841.5
Subject(s) - proteomics , transcriptome , biology , jasmonic acid , drought tolerance , gene , dna microarray , biochemistry , gene expression , botany
A peanut mini‐core accession relatively tolerant to drought was previously identified from our physiological screening. In the present study we employed a combined transcriptomics and proteomics analysis to study both the primary transcriptional networks and functional proteins involved in the drought tolerance mechanism. Peanut microarrays developed with 14352 genes in a high density (8 x 15k) format were used for transcript profiling. One‐ and two‐dimensional gel electrophoresis (1‐ and 2‐DGE) was performed on leaf soluble protein extracts of selected tolerant and susceptible accessions. Combined transcriptomics and proteomics analysis reveals that lipoxygenase, an enzyme of jasmonic acid biosynthesis induced under drought may be involved in drought signaling. Similarly, induction of the fatty acid biosynthesis enzyme, acetyl‐CoA carboxylase carboxyl transferase, in the tolerant accession suggests a possible role of lipid biosynthesis in the drought tolerance phenotype. Suppression of lectin proteins and induction of aldolases were also noticed and probably are consequences of general drought response in the tolerant peanut accession. Some of these identified proteins and their corresponding genes could contribute a physiological advantage under drought, making them potential candidates for marker‐assisted breeding.