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Regulation of Human Inducible Nitric Oxide Synthase by Protein‐protein Interaction
Author(s) -
Rashid Mohammad Bazlur,
Challawala Amama,
Chakravarty Deepavali,
Curtis Leigh,
Eissa N Tony
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.835.6
Subject(s) - gene isoform , nitric oxide , nitric oxide synthase , nos1 , microbiology and biotechnology , endothelial nos , biology , biochemistry , yeast , cell signaling , enzyme , chemistry , signal transduction , enos , gene , endocrinology
Nitric oxide (NO) is a potent cell‐signaling molecule that plays important roles in diverse range biological processes within various tissues. Almost in every cell of our body, NO is generated by certain enzymes called nitric oxide synthases (NOS). NO is synthesized constitutively in nervous systems and endothelial cells, whereas in some other cells NO production is induced. Excessive NO production is linked to carcinogenesis, septic shock, asthma, stroke, etc. On the other hand, insufficient NO is involved with hypertension, impotence, arteriosclerosis, etc. So, there are dual roles for NO molecule at different tissue level. Since three isoforms of NOS responsible for NO production, an isoform‐specific inhibitor(s) needs to be designed that would not interfere with another NOS. In this regard, we have employed BD‐Clontech made Yeast Two‐Hybrid System to identify the putative proteins that would specifically bind iNOS protein for regulation. An N‐terminal iNOS‐bait protein has been utilized to identify a mammalian cDNA library. Employing all sorts of negative and positive controls necessary to interpret Yeast Two‐hybrid data, we could identify a number protein that shows possible protein‐protein interaction with iNOS. The probable role of these proteins in the regulation or modulation of iNOS function will be discussed.