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Cofilin translocation to the nucleus is a key feature of decidualization
Author(s) -
Ihnatovych Ivanna,
Livak Mark,
Strakova Zuzana
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.824.2
Subject(s) - cofilin , microbiology and biotechnology , actin , chemistry , cytosol , actin cytoskeleton , myosin light chain kinase , decidualization , downregulation and upregulation , chromosomal translocation , phosphorylation , cytoskeleton , rho associated protein kinase , biology , biochemistry , cell , embryo , enzyme , gene
Decidualization (D), the differentiation of stromal fibroblasts (SF) into decidual cells, is critical for pregnancy establishment. D requires the reorganization of the actin cytoskeleton that is governed by phosphorylation (P) of myosin light chain and actin dynamics. Manipulation of actin dynamics by jasplakinolide prevents D of human SF. Actin dynamics are regulated by actin‐binding proteins, one of which is cofilin. The activity of cofilin depends on its P, which is regulated by LIMK1 and LIMK2. D of human SF is associated with changes in the distribution and activity of the proteins of the LIMK‐cofilin pathway. D induced by a stimulus of embryonic origin, interleukin‐1β (IL‐1β) and steroid hormones (H), is accompanied by an upregulation of LIMK1, a downregulation of LIMK2 and the translocation of inactivated (phosphorylated) cofilin from the nucleus into the cytosol. On the other hand, D induced by an artificial stimulus cAMP (with H) is characterized by a significant decrease in phosphorylated cofilin and unchanged amounts of LIMK1 and LIMK2. Despite the differences in molecular mechanisms underlying the D induced by H alone, IL‐1β+H or cAMP+H, the most striking observation during D is the translocation of cofilin from the cytosol to the nucleus. Taken together, the stabilization of F‐actin significantly inhibits D; and the translocation of cofilin to the nucleus is a key feature of D. Supported by HD044713