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Overexpression of YTCa, a novel gene can promote growth of NIH3T3 cells
Author(s) -
Hwa KuoYuan,
Tseng ChiaJen,
Cheng Wei,
Chang ChunChi,
Lee Yumay
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.819.3
Subject(s) - transfection , biology , gene , microbiology and biotechnology , messenger rna , complementary dna , differential display , cell , green fluorescent protein , genetics
YTCa, a novel human gene was first discovered in our studies for Dopa‐responsive dystonia by differential display. A unique cell model was established with the characteristic of the dominant‐negative effect of the GCH G201E mutation. By PCR‐based differential display, YTCa and other proteins were found to be involved in regulating cellular mRNA and protein half‐life. From bioinformatics analysis, we found that YTCa is located nearby chromosome 20q13. Although tissue distributions of YTCa mRNA were found in normal pancreas, muscle and heart, the protein can only be detected in brain by Western analysis. Moreover, normal tissue did not have detectable amounts of YTCa protein. In order to understand the functional role of YTCa, we have performed many experiments. First, overexpression of YTCa full‐length cDNA on NIH3T3 cell allowed cells to grow faster in tissue cultures under a low serum condition. Furthermore, nude mice injected with genetically altered NIH3T3 cells, stably transfected with YTCa formed tumors in 4 weeks. Acknowledgement: This work is supported by the grant from Academia Sincia, Taiwan, ROC (No: AS‐94‐TP‐B17).