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Gene profiling of the liver in high carbohydrate‐ and high fat diet‐fed mice unravels novel aspects of the disease
Author(s) -
Kirpich Irina,
Deaciuc Ion,
Song Zhenyuan,
McClain Craig
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.798.1
Subject(s) - fatty liver , gene , biology , disease , phenotype , nutrigenomics , gene expression profiling , gene expression , genetics , medicine
Alcoholic and non‐alcoholic fatty liver (NAFL) have similar phenotypical appearance, i. e. the presence of vacuolar fat in the hepatocyte. The most widespread diagnostic tool remains histological examination. New technologies such as gene profiling of tissues have brought new diagnostic tools for many diseases. We have performed gene profiling of NAFL of dietary origin in mice exposed to various diets and identified potential “signature genes”. Mice were exposed to two diets that produce NAFL: high carbohydrate (HCD) and high fat diet (HFD) which mimicked the composition of diets consumed by humans. At the killing, the animals displayed markers of metabolic syndrome and NAFL. Using Affymetrix and Agilent technology, we identified two major groups of genes that underwent expression changes. One group can be linked to the diet composition, called “adaptive genes”. The other, a much larger group, called “non‐adaptive genes” cannot be linked to the metabolic pathways of lipids or carbohydrates. These genes may be considered as consequential to NAFL. They unravel novel, never thought‐of aspects of the NAFL disease. Our experimental results allow a distinction to be made between NAFL of various dietary origins. Also, they may be suggestive for the clinician in an attempt to establish a diagnostic of the disease as a function of its origin.