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Relationship of the intravascular and pericellular (interstitial) oxygen pressures in normal and tumor tissue
Author(s) -
Wilson David F,
Vinogradov Sergei A,
Apreleva Sofia,
Lee William M F
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.794.14
Subject(s) - oxygen , chemistry , skeletal muscle , interstitial space , limiting oxygen concentration , oxygene , isoflurane , anatomy , biophysics , medicine , biology , organic chemistry
Objective: To determine the influence of vessel walls and diffusion distance in delivery of oxygen to cells in normal and K1735 murine melanoma tissue of mice. Methods: Oxygen pressures were measured by oxygen dependent quenching of phosphorescence, using oxygen probes contained in each compartment. Oxyphor G2 was added to the blood plasma and Oxyphor G3, Pd‐tetrabenzoporphyrin encapsulated with a gen. 2 poly‐arylglycine dendrimer and then coated with oligoethylene glycol residues (Av. MW 350), was microinjected into the interstitial (pericellular) space. The oxygen distributions in the two spaces were compared resting skeletal muscle and K1735 tumors in both awake and anesthetized mice. Results and conclusions: For skeletal muscle of awake mice, the lowest 10% of oxygen pressures for the interstitial and intravascular spaces (believed to represent capillary bed) were not different (23.8 ± 4.5 and 25 ± 4.3 mm Hg, respectively) whereas in isoflurane anesthetized mice there was a small but significant (p =0.01) difference (20.4 ± 6.3 and 27.9 ± 3.5 mm Hg, respectively). In K1735 tumors, compared to skeletal muscle, the oxygen pressures are more heterogeneous and averages lower. In addition there are larger differences between the two compartments, consistent with lower vascular density in the tumors. Supported by NS‐31465, HL081273, and U54 CA105008