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Transepithelial glucose transport and metabolism in H441 human airway epithelial cells
Author(s) -
Kalsi Kameljit Kaur,
Fraser Owen,
Chung YuenLi,
Tarelli Edward,
Philips Barbara,
Baker Emma,
Baines Deborah L
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.764.5
Subject(s) - metabolism , carbohydrate metabolism , apical membrane , glucose transporter , chemistry , glucose uptake , medicine , epithelium , endocrinology , epithelial polarity , compartment (ship) , mole , l glucose , biochemistry , biology , cell , diabetes mellitus , membrane , insulin , genetics , oceanography , geology , islet
Increased airway glucose is associated with increased risk of lung infection. The aim of this study was to characterise transepithelial glucose transport and metabolism in a model of human airway epithelial cells. Transepithelial transport of glucose from the basolateral compartment (B) to the apical compartment (A) or from A to B was traced using 3 H‐D‐glucose or non‐metabolizable 14 C‐L‐glucose. More apical radiolabel accumulated (B to A) than basolateral (A to B) with 5mM 3 H‐D‐glucose (0.103 ± 0.013 μmoles and 0.04 ± 0.01 μmoles per hour cm −2 p < 0.05, n = 4 respectively). Apical (B to A) and basolateral (A to B) radiolabel accumulation was similar with 5mM 14 C‐L‐glucose (0.03 ± 0.01 μmoles per hour cm‐ 2 and 0.03 ± 0.02 μmoles per hour cm‐ 2 respectively). However, HPLC analysis revealed that transepithelial apical glucose accumulation (B to A) was significantly less with 5mM D‐glucose (0.01 ± 0.02 μmoles per hour cm‐ 2 ) compared to 5mM L‐glucose (0.16 ± 0.05 μmoles per hour cm‐ 2 , p<0.05, n = 4). To try and account for this discrepancy we used proton NMR and identified metabolites formed from D‐glucose such as lactate, alanine, acetate and scyllo‐inositol (a phospholipid precursor) in the apical chamber. These data indicate that transepithelial transport and apical accumulation of glucose in the lung epithelium is reduced by intracellular metabolism and the production and release of glucose metabolites. Funded by Wellcome.