Premium
Hyperpolarized 3 He magnetic resonance imaging of ventilation after bacterial lipopolysaccharide exposure in mice
Author(s) -
Thomas Abraham,
Voltz James,
Fubara Boma,
Driehuys Bastiaan,
Zeldin Darryl
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.763.8
Subject(s) - medicine , lipopolysaccharide , distension , inhalation , ventilation (architecture) , lung , airway , magnetic resonance imaging , pathology , endocrinology , anesthesia , radiology , mechanical engineering , engineering
Traditional lipopolysaccharide (LPS) inhalation studies focus on histology, lung function and inflammatory mediator release. This study utilizes hyperpolarized (HP) 3 He MRI to elucidate regional differences in ventilation following LPS exposure in mice. Methods: C57BL/6 mice were intratracheally dosed with high dose LPS (50 μg, N=2), low dose LPS (15–30 μg, N=7) or normal saline (NS) (N=2) and then imaged by HP 3 He MRI. Results: There is proximal (2 nd or 3 rd order) bronchial tree distension in 50 μg LPS dosed mice that is not evident at lower doses (Table 1). Preliminary results indicate subsegmental ventilation defects (VD) are distributed evenly throughout the lungs of LPS dosed mice. Further, VD number is dose dependent (Table 1). Conclusions: LPS induced VD and proximal bronchial tree distension are dose dependent. As proximal bronchi distend in LPS dosed mice, airway hyperresponsiveness likely results from more distal airway constriction. Funding: NIEHS/NIH Division of Intramural Research and NCRR/NCI national resource funding.