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Compensatory hyperfiltration and NO in 2k1c and uninephrectomized rats
Author(s) -
Hultström Michael,
Helle Frank,
Iversen Bjarne M.
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.761.4
Subject(s) - kidney , medicine , endocrinology , contraction (grammar) , angiotensin ii , nitric oxide , renal function , renal blood flow , glomerular hyperfiltration , renal circulation , urology , chemistry , blood pressure , diabetic nephropathy
Afferent arterioles from the non‐clipped kidney from 2k1c rats has an increased nitric oxide (NO) release after angiotensin II stimulation. This study investigated if this could be compensatory hyperfiltration. Rats underwent uninephrectomy (unx), 2k1c‐ or sham‐surgery. Six weeks after the initial surgery the rats were sacrificed. The effect of L‐NAME on renal blood flow (RBF) was assessed with an ultrasound probe. Af were isolated using the agarose infusion method to study the effect of L‐NAME on Ang‐II induced contraction. NO release was studied using DAF‐FM. RBF was increased in the remnant kidney and in the non‐clipped kidney from 2k1c rats (3.82 ± 0.5 ml/min in controls compared to 8.0 ± 1.61 ml/min in the remnant kidney and 3.2 ± 0.17 and 4.8 ± 0.95 in the clipped and non‐clipped kidney respectively). L‐NAME treatment increased MAP around 30 mmHg in all groups while RBF decreased about 35% in all groups, except in the clipped kidney (−24 ± 3 %). AngII elicited similar contraction in Af from unx and controls. L‐NAME did not alter the contraction. No difference was seen in DAF fluorescence between Af from control and unx following AngII. In conclusion, our results in vivo indicate NO is of lesser importance in the clipped kidney than in the non‐clipped or in control kidneys. Unx Af does not have an increased AngII elicited NO release. The study was funded through Helsevest, Norway.