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Effect of ET‐1 on afferent arterioles of ET B ‐receptor deficient mice
Author(s) -
Schildroth Janice,
Sendeski Mauricio,
Rettig Juliane,
Steege Andreas,
Kalk Philipp,
Persson Pontus B.,
Hocher Berthold,
Patzak Andreas
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.761.20
Subject(s) - receptor , afferent arterioles , endocrinology , medicine , agonist , chemistry , vasoconstriction , blockade , afferent , biology , angiotensin ii
Endothelins (ET‐1, ET‐2, ET‐3) are a family of regulatory peptides that have long‐lasting vasoconstrictor and pressor effects. Among them ET‐1 is the most active form that plays an important role in the regulation of renal function. The aim of the study was to investigate the contribution of ET A ‐ and ET B ‐receptors in ET‐1 induced effects on afferent arterioles. Adult ET B ‐receptor deficient rescued mice and wild‐type mice were used. Cumulative dose‐response‐curves to ET‐1 (10 −12 to 10 −7 mol/L) were measured in isolated, perfused afferent arterioles, as well as during ET A ‐receptor blockade. Further, dose‐response curves to the ET B ‐receptor agonist 4‐Ala‐ET‐1 were measured. Results: 1. Afferent arterioles of wild‐type mice and ET B ‐receptor deficient mice showed a concentration‐dependent constriction to ET‐1. 2. The dose‐response curves did not differ between ET B ‐receptor deficient mice and wild‐type mice. 3. There was no response to ET‐1 during ET A ‐receptor blockade in afferent arterioles of both groups. 4. Afferent arterioles of wild‐type mice and ET B ‐receptor deficient mice did not significantly respond to 4‐Ala‐ET‐1. Conclusions: Results indicate that the ET A ‐receptor mediates vasoconstriction in afferent arterioles and that there is a lack of functional ET B ‐receptor activity in afferent arterioles.