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The divergent role of epoxyeicosatrienoic acids in the regulation of renal function in normotensive and two‐kidney one‐clip Goldblatt hypertensive rats
Author(s) -
Kopkan Libor,
Walkowska Agnieszka,
Thumova Monika,
Skaroupkova Petra,
Imig John D.,
Cervenka Ludek
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.761.1
Subject(s) - natriuresis , endocrinology , medicine , renal function , kidney , epoxygenase , renal blood flow , renovascular hypertension , chemistry , effective renal plasma flow , excretion , renal circulation , vasodilation , metabolism , cytochrome p450
To assess the renal function in anesthetized Hannover Sprague‐Dawley rats with induced renovascular hypertension for 27 days (2K1C) and normotensive sham operated control rats during acute inhibition of epoxyeicosatrienoic acids (EETs) generation in the kidney, epoxygenase inhibitor, methylsulfonyl‐6‐(2‐propargyloxyphenyl) hexanamide (MS‐PPOH, 12 μmol/L), was continuously administered directly into the nonclipped kidney at a rate 0.3 mL/hour for over 60 minutes. Glomerular filtration rate (GFR) and renal plasma flow (RPF) were determined by Inulin and PAH clearance, respectively. Basal renal function of the nonclipped kidney was not different (GFR 0.79±0.07 vs. 0.74 ±0.03 mL/min/g; RPF 2.03±0.09 vs. 2.04 ±0.13 mL/min/g and sodium excretion 1.85±0.31 vs. 1.51 ±0.29 μmol/min/g) in hypertensive 2K1C rats (n=8) compare to sham operated control rats (n=8). Although administration of MS‐PPOH caused significant decreases in GFR (15±3%), RPF (18±4%) and sodium excretion (42±9%) in sham operated rats, MS‐PPOH did not significantly alter renal function in the nonclipped kidney of 2K1C rats. These data further support the notion that EETs contribute in modulation of renal vasodilatation and natriuresis which might be impaired in 2K1C rats due to deficiency of intrarenal EETs in this hypertensive model.