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Nitric Oxide ‐ Red Blood Cells Interactions under Oxidative Stress
Author(s) -
Deonikar Prabhakar,
Kavdia Mahendra
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.758.38
Subject(s) - nitric oxide , superoxide , chemistry , oxidative stress , xanthine oxidase , nitrite , reactive oxygen species , biochemistry , hemoglobin , endothelium , endothelial dysfunction , pharmacology , endocrinology , biology , enzyme , organic chemistry , nitrate
Role of endothelium‐derived nitric oxide (NO) as a potent vasodilator has been well established. Homeostasis nitric oxide in vasculature is maintained by its production by endothelial nitric oxide synthase and consumption by red blood cells and smooth muscle cells. Endothelial dysfunction observed in pathophysiological conditions such as diabetes is characterized with increase in oxidative stress due to excessive production of reactive oxygen species like superoxide which contribute to the reduced nitric oxide bioavailability for vasodilation. In this study, we investigated the effect of oxidative stress on NO‐RBC interactions. For this purpose, a novel stirred bioreactor with controlled headspace NO concentration was designed. Oxidative stress conditions were generated using xanthine oxidase‐hypoxanthine system. Gaseous NO was reacted with RBC and superoxide for 10 min and samples were drawn every 2 min. Nitrite and total nitrogen species (NOx) concentrations in the samples were measured by chemiluminescence assay and hemoglobin derivatives were measure by spectrophotometric assay. Nitrite and total NOx concentrations for NO‐RBC interactions in presence of superoxide were lesser than that of NO‐RBC interaction in absence of superoxide. These results are important step towards understanding of endothelial cell dysfunction related bioavailability of NO.