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Pulmonary arterial oxidative stress in equine recurrent airway obstruction
Author(s) -
Venugopal Changaram,
Mariappan Nithya,
Elks Carrie,
Francis Joseph
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.758.14
Subject(s) - oxidative stress , pentoxifylline , reactive oxygen species , medicine , pathogenesis , tumor necrosis factor alpha , inflammation , endocrinology , pathology , pharmacology , immunology , chemistry , biochemistry
Oxidative stress in an important characteristic of hyperactive airways diseases such as recurrent airway obstruction (RAO) in horses. The primary source of oxidative stress in RAO is from the elevated amounts of reactive oxygen species (ROS) liberated from the inflammatory cells. ROS alters signal transduction mechanisms, activate redox‐sensitive transcription factors and regulate pro‐inflammatory gene expressions. Thus, ROS levels have important consequences for pathogenesis, severity and therapeutic management of RAO. The involvement of Tumor Necrosis Factor‐alpha (TNFα), a cytokine, is well established in inflammation. Therefore, the objective of the study was to determine oxidative stress in pulmonary arterial rings of RAO‐affected and unaffected horses and to test the effect of TNFα blockade using pentoxifylline. Arterial rings were collected from right diaphragmatic lung lobe and the ROS released by the tissues were measured using Electron Paramagnetic Resonance (EPR). The results showed that total ROS was significantly greater in RAO affected than the unaffected horses. In addition, incubation of the arterial rings with pentoxifylline reduced the ROS level in the RAO horses. The study suggested that pentoxifylline could be effective in allevieating oxidative stress in RAO. Supported by Equine Health Studies Program of LSU School of Veterinary Medicine.