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Distribution and expression of Aquaporin HC1 in tissues of Hyla chrysoscelis under different physiological conditions
Author(s) -
Pandey Ram Naresh,
Yaganti Sushmita,
Frisbie James,
Goldstein David L.
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.757.14
Subject(s) - immunohistochemistry , connective tissue , gastrointestinal tract , pathology , biology , kidney , anatomy , endocrinology , medicine , biochemistry
HC1 is an AQP1‐like water channel protein expressed by the treefrog Hyla chrysoscelis . Expression of HC1 mRNA is widespread among tissues. We used immunohistochemistry to localize HC1 protein within those tissues, and to explore variation in that expression in control (hydrated, 23C), dehydrated (20% loss of standard body mass over one week at 23C), and frozen (−2.5C for 24 hr) animals. Tissue sections were exposed to anti‐HC1 antibody and stained with Texas Red or Cy2 conjugated secondary antibody. In kidney, HC1 was detected in the parietal layer of the glomerulus and in overlying connective tissue. HC1 was expressed in glandular membrane in stratum spinosum of skin, in septa, sinusoids, and acini of liver, and in the gastrointestinal tract in gastric pits and in the lumen of crypts of small and large intestine. We also detected expression in the pulmonary epithelia and in blood vessels of several organs, including skeletal muscle and intestines. Our findings are largely consistent with the role of HC1 as a widely expressed “housekeeper” aquaporin. However, we also have preliminary evidence for some physiological regulation of expression, including down‐regulation in frozen liver and enhanced expression in skin from frozen and dehydrated animals. Research supported by NSF IOB‐0517301.