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Aquaporin and glyceroporin mRNA and protein expression in maturing Hyla chrysoscelis
Author(s) -
Wysong Robert,
Goldstein David L.
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.757.13
Subject(s) - aquaporin , biology , messenger rna , immunohistochemistry , blot , aquaporin 2 , microbiology and biotechnology , immunology , water channel , genetics , gene , mechanical engineering , engineering , inlet
Gray treefrogs Hyla chrysoscelis express aquaporins (HC1 and HC2) and glyceroporins (HC3) in tissue‐ and thermal‐specific patterns. These expression patterns likely facilitate water and glycerol redistribution during times of osmoregulatory challenge like dehydration or freezing. We hypothesized that expression of aquaporins would also change during growth and the transition from aquatic to terrestrial life. To test this, real time quantitative PCR and Western blotting were used to characterize the temporal expression of the three aquaporins, using beta actin as a baseline. At the earliest tadpole stage tested, Gosner developmental stage 24, mRNA expression was detectable for all three aquaporins in body tissue extracts. We tested ventral skin separately for expression of AQP‐HC2, which remained undetectable by Gosner stage 32, though it is present in adult frogs. Further experiments are underway to localize expression of these proteins to specific tissues using immunohistochemistry. Supported by NSF IOB‐0517301.