Moderate exercise training reduces kidney oxidative stress and mortality of hypertensive diabetic rats

Hypertension plays a negative role on the development of diabetic nephropathy, increasing the mortality of patients. Moderate exercise training decreases oxidative stress and is a therapy for cardiovascular and metabolic diseases. The aim was to determine the effect of exercise on renal oxidative stress and mortality of spontaneous hypertensive diabetic rats (SHR + streptozotocin 50mg/Kg; 3 months old) randomized into: sedentary (S, n=8) and trained (T, n=8) groups. After moderate exercise training (treadmill ‐ 1h/day, 5days/wk, 10wk), kidney was excised for measurement of oxidative damage (lipoperoxidation–LPO) and antioxidant enzymes activity (superoxide dismutase–SOD, catalase–CAT, and glutathione peroxidase–GPX activity) (Student's t‐test; Kaplan‐Meier method). T group presented lower renal LPO (T=1199± 184; S=2549± 111 counts/second/mg protein), higher renal antioxidant enzymes activity (SOD:T=10.6± 0.7; S=8.3± 0.4 U/mg protein; CAT:T=23722; S=164± 17 pmol/mg protein; GPX:T=7.8± 0.3; S=6.4± 0.5 nmol/min/mg protein; p<0.05) and lower mortality (p=0.03). Our results show that moderate exercise training reduces oxidative stress and suggests that this adaptation may decrease mortality of hypertensive diabetic animals. Financial Support: FAPESP, CNPq.