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Exercise‐induced hypoxia in rat skeletal muscle and tendon
Author(s) -
Skovgaard Dorthe,
Kjaer Andreas,
ElAli Henrik,
Kjaer Michael
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.753.27
Subject(s) - skeletal muscle , achilles tendon , tendon , hypoxia (environmental) , medicine , anatomy , stimulation , triceps surae muscle , chemistry , oxygenation , oxygen , organic chemistry
Studies have shown that intracellular oxygen in skeletal muscle decrease substantially during exercise, however less is known about the oxygenation of cells in the force‐transmitting tendons. The purpose of the present study was to test if Positron Emission Tomography (PET) with the hypoxia‐selective tracer 64 CuATSM can be applied for assessment of exercise‐induced hypoxia in rat skeletal muscle and tendon. Eight rats performed one‐leg contractions of the calf muscle by electro‐stimulation of the sciatic nerve. After ten minutes of muscle contractions 21.9±1.3 MBq 64 CuATSM was injected and contractions were continued for 20 minutes. Animals were scanned in a small animal PET/CT scanner and Standardized Uptake Values (SUVs) of 64 CuATSM in triceps surae muscle and Achilles tendons were obtained. Compared to resting conditions the uptake of 64 CuATSM was significantly increased in muscle ~1.5‐fold and in tendon ~1.3‐fold both immediately after cessation of contractions and 90 minutes post contractions (p< 0.05). The study shows that 64 CuATSM is irreversible trapped in hypoxic cells and can be used as a marker of transient exercise‐induced hypoxia in skeletal muscle and loaded tendons. Furthermore the study supports a decreased intracellular oxygen level in both skeletal muscle and the force‐transmitting tendon during muscle contractions.