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Markers of Mitochondrial Biogenesis following 3 days of Cycling
Author(s) -
Dumke Charles L,
Nieman David C,
Davis J Mark,
Murphy E. Angela,
Carmichael Martin D,
Henson Dru A,
Quindry John C,
Utter Alan C,
Triplett N. Travis,
McAnulty Steven R,
McAnulty Lisa S
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.753.19
Subject(s) - mitochondrial biogenesis , citrate synthase , cycling , endurance training , peroxisome , gene expression , mitochondrion , endocrinology , downregulation and upregulation , medicine , gene , coactivator , receptor , biology , enzyme , biochemistry , transcription factor , history , archaeology
Exercise increases mRNA for several genes involved in mitochondrial biogenesis. Less is known about how these genes respond to several successive days of intensive exercise. PURPOSE: To examine the effects of 3 h of intensive cycling over 3 successive days on gene expression in humans. METHODS: Twenty trained cyclists were tested for VO2max (54.7±1.1 ml.kg‐1.min‐1). The subjects cycled at 57% wattsmax for 3 h using their own bicycles on CompuTrainer™ Pro Model trainers (RacerMate, Seattle, WA) on 3 successive days. Muscle biopsies were obtained from the vastus lateralis pre‐ and post‐exercise on days 1 and 3. Muscle samples were analyzed (2(time) X 2(day) repeated measures ANOVA) for gene expression of cytochrome C, peroxisome proliferator receptor gamma coactivator‐1 alpha (PGC‐1A), citrate synthase, and Sirt‐1. RESULTS: Of the genes analyzed the following showed significant increases pre‐post 3 h rides: cytochrome C (P=0.03), PGC‐1A (P<0.001), and Sirt‐1 (P=0.05). The following genes showed significant effects from days 1 to 3: cytochrome C (P<0.001) and citrate synthase (P=0.009). CONCLUSION: Three successive days of intensive cycling resulted in upregulation of mRNA for mitochondrial enzymes and biogenesis markers in these trained subjects. These data contribute to the understanding of mitochondrial enzyme expression during prolonged exercise training.