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HIF‐1 specific prolyl hydroxylases in elite athletes
Author(s) -
Rundqvist Helene,
Klintberg Malene,
Norrbom Jessica,
Gustafsson Thomas,
Sundberg Carl Johan
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.753.11
Subject(s) - skeletal muscle , hydroxylation , elite athletes , medicine , hypoxia (environmental) , endocrinology , endurance training , hypoxia inducible factors , chemistry , biology , athletes , biochemistry , enzyme , gene , physical therapy , oxygen , organic chemistry
The hypoxia sensitive transcription factor HIF‐1 has been suggested to be involved in skeletal muscle adaptation to endurance‐type exercise training. HIF‐1 protein levels are regulated by hydroxylation of critical proline residues and subsequent protein degradation of the alpha subunit. Three prolyl hydroxylation domain‐containing proteins (PHD1‐3) have been identified in mammalian cells, PHD2 appears to be the predominant form that regulates HIF‐1alpha protein stability in vivo 1 . HIF‐1 is stabilised in response to an acute bout of exercise in sedentary individuals 2 but there is debate regarding the activity of the HIF‐1 system in response to long term training. Skeletal muscle biopsies were obtained from the vastus lateralis muscle at rest from 12 males with high oxidative capacity and 9 normally trained male controls. PHD2 mRNA displayed the highest expression levels of the three forms in human skeletal muscle, regardless of training status. Furthermore, PHD2 and 3 mRNA levels were both higher in elite athletes compared to the control individuals and PHD2 protein levels were significantly higher in the elite group. Conclusion: The HIF‐1 regulating prolyl hydroxylase PHD2 was significantly higher in elite athletes, which may explain an attenuated HIF‐1 activity in response to long term endurance training. This work was supported by the Swedish Research Council, the Swedish National Centre for Research in Sports