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Hyperglycemia induced oxidative stress is attenuated in cardiac tissue by 670nm photobiomodulation in an experimental model of diabetes
Author(s) -
Gopalakrishnan Sandeep,
Lark Daniel S,
Sandoval Chris L,
Snyder Ann C,
Eells Janis T
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.751.5
Subject(s) - oxidative stress , diabetic cardiomyopathy , streptozotocin , diabetes mellitus , medicine , glutathione , endocrinology , chemistry , cardiomyopathy , biochemistry , heart failure , enzyme
Oxidative stress contributes to the pathogenesis of diabetic cardiomyopathy. Photobiomodulation by light in the red to near‐infrared (NIR) range (630–1000 nm) using low energy lasers or light‐emitting diode (LED) arrays accelerates wound healing, improves recovery from ischemic injury and attenuates degeneration in the injured optic nerve. Purpose To investigate the efficacy of 670 nm NIR‐LED treatment in attenuating diabetes‐induced oxidative stress in cardiac tissue. Methods Adult Wistar rats were made diabetic by streptozotocin treatment (STZ, 50 mg/kg, ip). One group of animals (NIR‐STZ) was treated daily with 670 nm LED irradiation. (5 min/day, 6 J/cm 2 ) 5 days/week for 14 weeks. Other treatment groups were untreated control (CON) and STZ‐treated (STZ). At week 14, animals were euthanized, perfused with cold PBS and the heart was rapidly harvested and flash frozen. Glutathione concentrations were determined by an enzymatic recycling method. Results The GSSG/GSH ratio in heart tissue was increased nearly four fold in STZ‐treated rats relative to control animals (CON = 0.08 ±0.01, vs. STZ=0.31± 0.08). 670 nm LED treatment significantly attenuated GSH oxidation (NIR‐STZ = 0.13± 0.03). Conclusion NIR photobiomodulation reduces intracellular oxidative stress and may attenuate cardiomyopathy in diabetes. Funding Source Biomedical Technology Alliance Award and UWM Research Growth Initiative