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Resveratrol attenuates cigarette smoking‐induced pro‐inflammatory alterations in the endothelial phenotype
Author(s) -
Ungvari Zoltan,
Labinskyy Nazar,
Pacher Pal,
Zhang Cuihua,
Podlutsky Andrej,
Csiszar Anna
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.747.4
Subject(s) - resveratrol , inflammation , oxidative stress , sirtuin 1 , endothelial dysfunction , chemistry , pharmacology , endothelium , endocrinology , proinflammatory cytokine , medicine , biochemistry , downregulation and upregulation , gene
Previously we have shown that water soluble components of cigarette smoke (CS) up‐regulate NAD(P)H oxidases and activate NF‐kB leading to vascular inflammation, likely increasing increased cardiovascular risk in smokers. To determine whether the sirtuin activator resveratrol (RES) protects endothelial cells against the deleterious effects of CS, rats were exposed to the smoke of 5 cigarettes per day (for one week, with or without RES treatment). CS impaired acetylcholine‐induced relaxations of carotid arteries, which could be improved by RES treatment. Both smoking and in vitro treatment of coronary arterial endothelial cells (CAECs) with CS extract significantly increased endothelial O2.‐ production, which were attenuated by RES. Expression of the pro‐inflammatory cytokines (IL‐1, IL‐6 and TNFa) and that of iNOS was significantly increased by CS, which could be attenuated by RES treatment. In CAECs CS treatment elicited NF‐kB activation and increased monocyte adhesiveness, which were prevented by RES. Thus, RES at nutritionally relevant concentrations attenuates CS‐induced oxidative stress, improves endothelial function and inhibits vascular inflammation.