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A motion‐based angiogenesis analysis to quantify new blood vessel growth
Author(s) -
Tong Edmund Y.,
Collins Geoffrey C.,
Judkins Kyle M.,
GreeneColozzi April E.,
Chen Julia L.,
Lee Joseph A.,
Manos Philip D.
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.746.19
Subject(s) - zebrafish , magnification , angiogenesis , danio , fish fin , anatomy , biomedical engineering , blood vessel , biology , fish <actinopterygii> , medicine , computer science , computer vision , biochemistry , cancer research , fishery , gene , endocrinology
We developed a new method to quantify blood vessel growth using a short video recording of the vascular bed in the regenerated region of the zebrafish caudal fin. After cutting the three most ventral caudal fin bones at the point of branching, each zebrafish, Danio rerio, was exposed to an angiogenic factor for 60 minutes. On day six post amputation, after each fish was anesthetized with 0.2% Tricaine, the newly developed vascular bed in the regenerated area was examined under a Nikon microscope at 40X magnification and a 15 second video clip was recorded. The last 20 frames of the clip were then imported to an imaging software program called Environment for Visualizing Images 3.6 (ENVI 3.6). The only difference among these 20 frames was the movement of blood through all the blood vessels. Consequently, the deviation among the frames highlighted only the blood vessels. When only the newly developed vascular bed in the regenerated area was outlined, a precise, objective and easy comparison could be made between the angiogenic response in control and experimental zebrafish. The same method may be used to quantify angiogenic effects in other models with some modifications.