Premium
FITC localization of renin in rat mesometrial tissue
Author(s) -
Hurley Mary Eder,
Quarshie Rudrick,
Gibrill Yusif,
Critelli Marta,
Hurwitz Melissa,
Gurita Cristian,
Olivieri Marion Pace
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.735.13
Subject(s) - afferent arterioles , trophoblast , renin–angiotensin system , placenta , biology , kidney , medicine , endocrinology , fetus , anatomy , blood pressure , pregnancy , genetics
Renin, a hormone produced primarily by the kidneys in response to low blood pressure, is an immediate downstream gene product of Class I HOX gene regulation in the embryo. Examination of transgenic mice in which renin was linked to gfp reveals renin in renal, adrenal, and gonadal arteries in the embryo, giant trophoblast cells of the placenta, kidney and submandibular gland in both male and female, and uterine artery. In rat, giant trophoblast cells, other cells in fetal and maternal regions of the placenta, and cells of the tunica adventita of uterine artery as well as in the wall of the afferent arteriole of the kidney were labeled with antibody stain. This project examines pregnant and nonpregnant rat mesometrial tissues to identify cells that localize FITC labeled sheep anti‐rat renin antibody. Fluorescent granules were seen in cells resembling fibroblasts throughout the mesometrium and along the walls of small vessels within the mesometrium. Afferent arterioles were labeled and control tissues were not. These results demonstrate that the mesometrium is another site within rat female reproductive tissues in which renin is located, adding support to the hypothesis that extrarenal renin production may play a role in preecclampsia. Work is supported by NSF grant #0132823 and 0726185, and D’Youville College faculty council research funding.