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Evaluating criteria for intact capillaries in Rana pipiens using hydraulic conductivity (L p ), rolling and sticking white blood cells (WBC), and an index of systemic inflammation
Author(s) -
Williams Donna A.,
WipkeTevis Deidre D.,
Flood Mary H.
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.731.4
Subject(s) - rana , medicine , inflammation , endocrinology , basal (medicine) , white blood cell , chemistry , andrology , zoology , biology , anatomy , insulin
WBC rolling/sticking along capillary walls have been used for decades to identify inflammation and exclude microvessels from studies of basal capillary function in vivo . For microcirculatory preparations a broader view of animal health state also is required, but often remains undefined. We measured activation of WBC obtained from a peripheral blood sample as an index of systemic inflammation in Rana pipiens and then measured L p on individual capillaries always devoid of rolling/sticking WBC. We hypothesized that capillary L p following our standard protocol (abrupt change in shear stress (Δτ)) would be higher in frogs with activated WBC (oxidative burst) compared to control. Frogs ( Rana pipiens , N =38) were pithed and mesentery was exteriorized and superfused with frog Ringer's (14–16°C). True capillaries were cannulated at 10 cm H 2 O, perfused with 10 mg·ml −1 BSA/Ringer's, stimulated with Δτ, and L p assessed at 30 cm H 2 O (modified Landis technique). Mean±SE L p for control capillaries (no activated WBC; within 95% confidence intervals (CI) of Δτ/L p response curve) was 2.7±0.3 × 10 −7 cm·s −1 ·cm H 2 O −1 (n=15). In frogs with activated WBC, L p was higher (11.4±1.6 × 10 −7 , n=9; P <0.0001) compared to control. A subset of frogs with no activated WBC displayed L p values outside CI that averaged 8.9±1.1 × 10 −7 (n=12), were higher than control, and did not differ from the activated WBC group. These data suggest that capillary rolling/sticking WBC may not provide comprehensive information concerning inflammation of intact capillaries in Rana pipiens . Systemic activated WBC accounted for half of the identified outliers. Supported by RO1 HL63125.