Premium
PACE therapy and its influence on microcirculatory hemodynamics and leukocyte‐endothelial interactions
Author(s) -
Krokowicz Lukasz,
Klimczak Alesandra,
Duggan William,
Grykien Christopher,
Mielniczuk Mariusz,
Siemionow Maria
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.731.16
Subject(s) - downregulation and upregulation , hemodynamics , medicine , immunohistochemistry , pace , cremaster muscle , cell adhesion molecule , microcirculation , endocrinology , immunology , chemistry , biochemistry , gene , geodesy , geography
Purpose: Effect of Pulsed Acoustic Cellular Therapy (PACE) therapy on muscle microcirculatory hemodynamics, neovascularization, leukocyte‐endothelial interactions and muscle flap healing was assessed. Methods: Cremaster muscles were dissected in 42 Lewis rats, divided into 5 groups: 1) controls (n=10); 2) PACE with 200 or 3) 500 impulses (n=8 each) immediately before dissection; 4) PACE with 200 or 5) 500 impulses (n=8 each) 24h before dissection, followed by microcirculatory hemodynamic recording at 1,2,3,4 h. Immunohistochemistry assessed expression of cell adhesion molecules and proangiogenic factors: VEGF and vWF. Results: PACE increased diameter of first (A‐1 in group 3) and second order arterioles (A‐2 in groups 2 and 3) (P<0.05). In groups 2, 3 and 4 rolling and sticking leukocytes were significantly decreased compared to controls (P<0.05). 200 impulses of PACE increased number of transmigrating leukocytes compared to 500 PACE impulses and to controls (P<0.05). Leukocyte‐endothelial interaction correlated with upregulation of ELAM‐1, CD15s and VCAM‐1. Synergistic expression of proangiogenic factors was significantly upregulated 24h after 500 impulses of PACE. Conclusions: PACE therapy increased main arterioles diameters and has direct effect on activation of leukocyte‐endothelial interactions and stimulation of expression of proangiogenic factors at microcirculatory levels