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Local inflammatory response to intraperitoneal (ip) instrumentation
Author(s) -
Flessner Michael F,
Credit Kimberly,
Henderson Karla,
He Zhi,
Li Xiaorong,
Potter Rebecca,
GomezSanchez Elise
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.731.12
Subject(s) - peritoneum , peritoneal cavity , masson's trichrome stain , medicine , peritonitis , angiogenesis , catheter , abdominal cavity , pathology , silicone , mannitol , staining , surgery , chemistry , organic chemistry
After observing cytokine staining in the peritoneum of rats with ip transmitters and aortic catheters, we hypothesized that foreign materials in the cavity cause inflammation. To address this, we aseptically implanted medical grade silicone catheters in the peritoneal cavity of rats. After 1–4 weeks, we cultured the catheter to rule out peritonitis and measured the osmotic volume flux (J osm ) and the mannitol mass transfer coefficient (MTC) using plastic chambers affixed to the abdominal wall (AW) peritoneum for 120 min in 12 catheter animals (CATH) and 6 age controls (CON). Peritoneal thickness was measured in trichrome‐stained AW, while angiogenesis was determined with CD31 stain and an optical micrometer. VEGF, FGF, TGFβ showed marked staining in CATH animals with ~none in CON. Large differences (p<.02) were observed in peritoneal thickness (mm±SE: CATH, 267±55 vs CON, 19±3), angiogenesis (#ves/mm±SE: CATH, 51±3 vs CON, 0±0), and MTCs (cm/min × 10 3 ± SE: CATH, 3.97±.32 vs CON, 2.52±.46), but J osm were not different. We conclude that medical grade silicone in the peritoneal cavity sets up a significant inflammatory response that alters the structure and transport function of the peritoneum.